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pcDNA3-Xl-VSP1 R152Q
(Plasmid #51881)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51881 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression ; Xenopus oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VSP1 R152Q
  • Alt name
    tpte2
  • Alt name
    Voltage Sensitive Phosphatase 1
  • Species
    X. laevis (frog)
  • Mutation
    R152Q: near the extracellular side of the fourth transmembrane domain, shifts Xl-VSP1 phosphatase activation to more negative membrane potentials
  • Entrez Gene
    tpte2
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Xl-VSP1 R152Q was a gift from Laurinda Jaffe (Addgene plasmid # 51881 ; http://n2t.net/addgene:51881 ; RRID:Addgene_51881)

  • For your References section:

    Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence. Ratzan WJ, Evsikov AV, Okamura Y, Jaffe LA. J Cell Physiol. 2011 Nov;226(11):2740-6. doi: 10.1002/jcp.22854. 10.1002/jcp.22854 PubMed 21618529
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