pLPC-Flag-CHES1L-dC-NLS
(Plasmid
#51840)
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PurposeFlag-CHES1L mutant lacking the C-terminal, with remaining NLS
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLPC
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCHES1
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Alt nameFOXN3
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SpeciesH. sapiens (human)
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Mutationcontains CHES1 amino acids 1-205
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Entrez GeneFOXN3 (a.k.a. C14orf116, CHES1, PRO1635)
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- NLS (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPC-Flag-CHES1L-dC-NLS was a gift from Gerardo Ferbeyre (Addgene plasmid # 51840 ; http://n2t.net/addgene:51840 ; RRID:Addgene_51840) -
For your References section:
CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and protein biosynthesis. Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, Gaumont-Leclerc MF, Moro A, Ferbeyre G. Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. Epub 2014 Jan 8. 10.1091/mbc.E13-02-0110 PubMed 24403608