pExp_TRE:EBFP2
(Plasmid
#51793)
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PurposeInducible expression of EBFP2 with rtTA for input fluorescence
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZDONOR 5-6
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEBFP2
- Promoter TRE
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCEP-fwd
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pExp_TRE:EBFP2 was a gift from Ron Weiss (Addgene plasmid # 51793 ; http://n2t.net/addgene:51793 ; RRID:Addgene_51793) -
For your References section:
Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition. Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R. ACS Synth Biol. 2014 Nov 17. 10.1021/sb500263b PubMed 25369267