pRS327
(Plasmid #51787)

• Purpose: (Empty Backbone) multicopy (YEp) vector with a LYS2 marker and a 2-μm origin of replication
• Depositing Lab: David Stillman
• Publication: Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

Backbone

• Vector backbone: pRS423
• Modifications to backbone: The CEN plasmid pRS327 was constructed by homology mediated recombination in yeast. A 1.84-kb ApaLI fragment from pRS423 that has the 2-μm origin of replication was transformed into lys2 yeast along with plasmid pRS307 that had been linearized with AatII. Selection for Lys+ prototrophs identified yeast where recombination of the homologous regions between the two plasmids created a circular DNA molecule with the 2-μm origin of DNA replication and the LYS2 marker.
• Vector type: Yeast Expression
• Selectable markers: LYS2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry