pRS307
(Plasmid #51785)

• Purpose: (Empty Backbone) an integrating (YIp) vector with a LYS2 selectable marker
• Depositing Lab: David Stillman
• Publication: Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

Backbone

• Vector backbone: pRS306 and pRS317
• Modifications to backbone: pRS317 was cleaved at the ApaLI site common to the pRS vectors, the ends were blunted with the Klenow fragment of DNA polymerase, the DNA was cleaved with EcoRI, and the 5.57-kb ApaLI (blunt)/EcoRI fragment was purified. pRS306 was cleaved with NdeI, the ends were blunted with the Klenow fragment of DNA polymerase, cleaved with EcoRI, and the 2.65-kb NdeI (blunt)/EcoRI fragment was purified. The two fragments were ligated to create pRS307.
• Vector type: Yeast Expression
• Selectable markers: LYS2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry