RP1B-PP1gamma
(Plasmid
#51770)
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PurposeExpresses PP1 gamma residues 7-323
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51770 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRP1B
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Backbone manufacturerPeti Lab (Addgene Plasmid# 26563)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAlpha-Select Gold Efficiency cells from Bioline are recommended for expression
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepp1 gamma
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MutationContains PP1 gamma residues 7-323
- Promoter T7-lac
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Tags
/ Fusion Proteins
- Thio-6 (N terminal on backbone)
- His (N terminal on backbone)
- TEV protease site (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RP1B-PP1gamma was a gift from Wolfgang Peti (Addgene plasmid # 51770 ; http://n2t.net/addgene:51770 ; RRID:Addgene_51770)