Skip to main content
Addgene

RP1B-PP1alpha
(Plasmid #51768)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51768 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    RP1B
  • Backbone manufacturer
    Peti Lab (Addgene Plasmid# 26563)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Alpha-Select Gold Efficiency cells from Bioline are recommended for expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pp1 alpha
  • Mutation
    Contains PP1 alpha residues 7-330
  • Promoter T7-lac
  • Tags / Fusion Proteins
    • Thio-6 (N terminal on backbone)
    • His (N terminal on backbone)
    • TEV protease site (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RP1B-PP1alpha was a gift from Wolfgang Peti (Addgene plasmid # 51768 ; http://n2t.net/addgene:51768 ; RRID:Addgene_51768)

  • For your References section:

    Crystal structures of protein phosphatase-1 bound to nodularin-R and tautomycin: a novel scaffold for structure-based drug design of serine/threonine phosphatase inhibitors. Kelker MS, Page R, Peti W. J Mol Biol. 2009 Jan 9. 385(1):11-21. 10.1016/j.jmb.2008.10.053 PubMed 18992256
Related items:
Commonly requested with: