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pCDNA3 T7 Jip1(471-660)
(Plasmid #51766)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jnk interacting protein 1
  • Alt name
    Jip 1(471-660)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    615
  • Mutation
    deleted 1-470
  • GenBank ID
    AF003115.1
  • Entrez Gene
    Mapk8ip1 (a.k.a. IB1, JIP-1, Jip1, Prkm8ip, Skip, mjip-2a)
  • Tag / Fusion Protein
    • T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 T7 Jip1(471-660) was a gift from Roger Davis (Addgene plasmid # 51766 ; http://n2t.net/addgene:51766 ; RRID:Addgene_51766)

  • For your References section:

    A cytoplasmic inhibitor of the JNK signal transduction pathway. Dickens M, Rogers JS, Cavanagh J, Raitano A, Xia Z, Halpern JR, Greenberg ME, Sawyers CL, Davis RJ. Science. 1997 Aug 1. 277(5326):693-6. 10.1126/science.277.5326.693 PubMed 9235893
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