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Addgene

peGFPC2-CARKL
(Plasmid #51748)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-C2
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHPK
  • Alt name
    CARKL
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1431
  • Entrez Gene
    Shpk (a.k.a. RP23-214D6.1, 4930431K22Rik, AI194947, AW260459, Carkl)
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer eGFP
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peGFPC2-CARKL was a gift from Markus Ralser (Addgene plasmid # 51748 ; http://n2t.net/addgene:51748 ; RRID:Addgene_51748)

  • For your References section:

    The pentose phosphate pathway is a metabolic redox sensor and regulates transcription during the antioxidant response. Kruger A, Gruning NM, Wamelink MM, Kerick M, Kirpy A, Parkhomchuk D, Bluemlein K, Schweiger MR, Soldatov A, Lehrach H, Jakobs C, Ralser M. Antioxid Redox Signal. 2011 Jul 15;15(2):311-24. doi: 10.1089/ars.2010.3797. Epub 2011 May 19. 10.1089/ars.2010.3797 PubMed 21348809
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