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Addgene

p413TEF-Sir2-P394L
(Plasmid #51744)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51744 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p413TEF
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sir2
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    P394L
  • Entrez Gene
    SIR2 (a.k.a. YDL042C, MAR1)
  • Promoter TEF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer TEF1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p413TEF-Sir2-P394L was a gift from Markus Ralser (Addgene plasmid # 51744 ; http://n2t.net/addgene:51744 ; RRID:Addgene_51744)

  • For your References section:

    Interfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. Ralser M, Zeidler U, Lehrach H. PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24. 10.1371/journal.pone.0005376 PubMed 19390637
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