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Addgene

pSplit-OC-U5-40K
(Plasmid #51741)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51741 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4620
  • Total vector size (bp) 6180
  • Modifications to backbone
    MfeI-XhoI fragment of U5-40K was cloned into the EcoRI and XhoI sites, then U5-40K cannot be recovered by enzyme digestion.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U5-40K
  • Species
    H. sapiens (human)
  • Entrez Gene
    SNRNP40 (a.k.a. 40K, HPRP8BP, PRP8BP, PRPF8BP, SPF38, WDR57)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Luciferase (394-550aa) (C terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSplit-OC-U5-40K was a gift from Hiroyoshi Ariga (Addgene plasmid # 51741 ; http://n2t.net/addgene:51741 ; RRID:Addgene_51741)

  • For your References section:

    A split luciferase-based reporter for detection of a cellular macromolecular complex. Maita H, Tomita K, Ariga H. Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 10.1016/j.ab.2014.01.015. 10.1016/j.ab.2014.01.015 PubMed 24503441
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