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Addgene

R5 EMCV
(Plasmid #51733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGl4.13
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 6208

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EMCV IRES
  • Insert Size (bp)
    583

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer TTCTAGAGCTTATCGATACC
  • 3′ sequencing primer TGAAGGAGTCCAGCACGTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R5 EMCV was a gift from Vincent Mauro (Addgene plasmid # 51733 ; http://n2t.net/addgene:51733 ; RRID:Addgene_51733)

  • For your References section:

    Analysis of rRNA processing and translation in mammalian cells using a synthetic 18S rRNA expression system. Burman LG, Mauro VP. Nucleic Acids Res. 2012 Sep;40(16):8085-98. doi: 10.1093/nar/gks530. Epub 2012 Jun 20. 10.1093/nar/gks530 PubMed 22718970
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