pDEST myc MOV10 D645N
(Plasmid
#51720)
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Purposeplasmid expresses myc-tagged MOV10 D645N
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST26
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5882
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMOV10 D645N
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SpeciesH. sapiens (human)
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Insert Size (bp)3012
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MutationD645N
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GenBank IDNP_001123551
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Entrez GeneMOV10 (a.k.a. fSAP113, gb110)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST myc MOV10 D645N was a gift from Markus Landthaler (Addgene plasmid # 51720 ; http://n2t.net/addgene:51720 ; RRID:Addgene_51720) -
For your References section:
MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs. Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa S, Dieterich C, Landthaler M. Mol Cell. 2014 May 22;54(4):573-85. doi: 10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10. 10.1016/j.molcel.2014.03.017 PubMed 24726324