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Addgene

pFRT/FLAG/HA MOV10 K530A
(Plasmid #51716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT/FLAG/HA-DEST
  • Backbone size w/o insert (bp) 5299
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MOV10 K530A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3012
  • Mutation
    K530A
  • GenBank ID
    NP_001123551
  • Entrez Gene
    MOV10 (a.k.a. fSAP113, gb110)
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/FLAG/HA MOV10 K530A was a gift from Markus Landthaler (Addgene plasmid # 51716 ; http://n2t.net/addgene:51716 ; RRID:Addgene_51716)

  • For your References section:

    MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs. Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa S, Dieterich C, Landthaler M. Mol Cell. 2014 May 22;54(4):573-85. doi: 10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10. 10.1016/j.molcel.2014.03.017 PubMed 24726324
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