pCDNA3-ySet2
(Plasmid #51698)

• Purpose: Expresses full length yeast Set 2 gene in a mamalian expression vector
• Depositing Lab: Tom Misteli
• Publication: Luco et al Science. 2010 Feb 19;327(5968):996-1000. doi: 10.1126/science.1184208. Epub 2010 Feb 4.

Backbone

• Vector backbone: pcDNA3.1 +
• Backbone manufacturer: commercial
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7600
• Modifications to backbone: none
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: yeast Set2
• Alt name: SETD2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2200
• GenBank ID: NC_001142.9 NM_001181601
• Entrez Gene: SET2 (a.k.a. YJL168C, EZL1, KMT3)
• Promoter: CMV
• Tag / Fusion Protein:
  • C-terminal FLAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • BAC387_ySet2_flag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry