M4786 KanMX::hisG-URA3-HisG Disruptor Converter
(Plasmid #51690)

• Purpose: Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG
• Depositing Lab: David Stillman
• Publication: Voth et al Yeast. 2003 Aug;20(11):985-93.

Backbone

• Vector backbone: M4757 (kanMX::TRP1), Addgene plasmid #51687
• Vector type: Yeast Expression ; yeast marker swap
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hisG-URA3-hisG
• Alt name: URA3
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: URA3 (a.k.a. YEL021W)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

M4786 (kanMX::hisG-URA3-hisG) was made by digesting M4757 with BamHI and SalI and inserting a 3.9 kb BamHI–SalI fragment with hisG–URA3–hisG from plasmid M2524.

The kanMX::hisG–URA3–hisG converter is recyclable, so that any MX-based disruption can be reverted to an unmarked disruption, allowing reuse of markers.