M4757 KanMX::TRP1 Disruptor Converter
(Plasmid #51687)

• Purpose: Yeast marker swap plasmid for converting KanMX3 to TRP1
• Depositing Lab: David Stillman
• Publication: Voth et al Yeast. 2003 Aug;20(11):985-93.

Backbone

• Vector backbone: pAG25 (Goldstein and McCusker, 1999)
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TRP1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: TRP1 (a.k.a. YDR007W)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

First, plasmid pAG25 (Goldstein and McCusker, 1999) was digested with HindIII and PacI, treated with Klenow and ligated closed. This plasmid was digested with KpnI and SphI and the 0.9 kb KpnI–SphI fragment containing TRP1 from pJJ246 was inserted, constructing M4757.