M4753 KanMX::ADE2 Disruptor Converter
(Plasmid #51683)

• Purpose: Yeast marker swap plasmid for converting KanMX3 to ADE2
• Depositing Lab: David Stillman
• Publication: Voth et al Yeast. 2003 Aug;20(11):985-93.

Backbone

• Vector backbone: M4757 (kanMX::TRP1), Addgene plasmid # 51687
• Vector type: Yeast Expression ; yeast marker swap
• Selectable markers: ADE2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ADE2
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: ADE2 (a.k.a. YOR128C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: sp6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

M4753 (kanMX::ADE2) was made by digesting M4757 with KpnI and SalI and inserting a 2.3 kb KpnI–SalI fragment with ADE2 from plasmid pFA6–ADE2.