M3925 trp1::KanMX3 Disruptor Converter
(Plasmid
#51681)
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PurposeYeast marker swap plasmid for converting Trp1 to KanMX3
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYDp-W
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Vector typeYeast Expression ; yeast marker swap
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKanMX3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid M3925 (trp1::KanMX3) was made by a three-fragment ligation, using a 3538 bp SmaI fragment from YDp-W, a 726 bp SmaI–MluI fragment from pFA6–KanMX3 and a 1774 bp MluI–EcoRV fragment from
pFA6–KanMX3.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
M3925 trp1::KanMX3 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51681 ; http://n2t.net/addgene:51681 ; RRID:Addgene_51681) -
For your References section:
New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713
Map uploaded by the depositor.
