M2660 ura3::LYS2 Disruptor Converter
(Plasmid #51678)

• Purpose: Yeast marker swap plasmid for converting URA3 to LYS2
• Depositing Lab: David Stillman
• Publication: Voth et al Yeast. 2003 Aug;20(11):985-93.

Backbone

• Vector backbone: M581
• Backbone manufacturer: Marsh et al., 1984
• Vector type: Yeast Expression ; yeast marker swap
• Selectable markers: LYS2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LYS2
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: LYS2 (a.k.a. YBR115C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid M2660 (ura3::LYS2 ) was made by cutting M581 with EcoRV and PstI and replacing the 208 bp EcoRV–PstI fragment with a 4.6 kb PvuII–PstI fragment containing LYS2 from plasmid YDp-K.