M3499 ura3::ADE2 Disruptor Converter
(Plasmid #51674)

• Purpose: Yeast marker swap plasmid for converting URA3 to ADE2
• Depositing Lab: David Stillman
• Publication: Voth et al Yeast. 2003 Aug;20(11):985-93.

Backbone

• Vector backbone: pJJ244
• Total vector size (bp): 7335
• Vector type: Yeast Expression ; yeast marker swap
• Selectable markers: ADE2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ADE2
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: ADE2 (a.k.a. YOR128C)
• Promoter: ADE2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: StuI (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid M3499 (ura3::ADE2 ) was made by cutting pJJ244 with StuI and EcoRV and replacing the 248 bp StuI–EcoRV fragment with a 3.6 kb BamHI blunted with Klenow) fragment containing ADE2 from plasmid M1144.