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M3939 leu2::ADE2 Disruptor Converter
(Plasmid #51672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51672 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YDpL
  • Total vector size (bp) 8058
  • Vector type
    Yeast Expression ; yeast marker swap
  • Selectable markers
    ADE2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ADE2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ADE2 (a.k.a. YOR128C)
  • Promoter ADE2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid M3939 (leu2::ADE2) was made by cutting YDp-L with EcoRV, and inserting a 3.6 kb BamHI (blunted with Klenow) fragment with ADE2 containing plasmid M1144.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M3939 leu2::ADE2 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51672 ; http://n2t.net/addgene:51672 ; RRID:Addgene_51672)

  • For your References section:

    New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713
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