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Purpose(Empty Backbone) yeast plasmid for integration of target sequence at HO locus, URA3 selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51663 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneHO-Poly-HO
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Backbone manufacturerStillman Lab, Addgene plasmid # 51661
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Modifications to backbonePlasmid HO-hisG-URA3-hisG-poly-HO was constructed by inserting the 3853 bp BamHI–BglII fragment with the hisG-URA3-hisG cassette (Alani, et al. 1997) into BamHI digested HO-poly-HO.
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Vector typeYeast Expression ; yeast integration
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HO-hisG-URA3-hisG-poly-HO was a gift from David Stillman (Addgene plasmid # 51663 ; http://n2t.net/addgene:51663 ; RRID:Addgene_51663) -
For your References section:
Yeast vectors for integration at the HO locus. Voth WP, Richards JD, Shaw JM, Stillman DJ. Nucleic Acids Res. 2001 Jun 15;29(12):E59-9. 10.1093/nar/29.12.e59 PubMed 11410682
Map uploaded by the depositor.
