HO-Poly-HO
(Plasmid #51661)

• Purpose: (Empty Backbone) yeast plasmid for integration of target sequence at HO locus
• Depositing Lab: David Stillman
• Publication: Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.

Backbone

• Vector backbone: pUC21-NotI
• Backbone manufacturer: Stillman Lab, Addgene plasmid # 51659
• Modifications to backbone: A 912 bp fragment (HO-L) with sequences from the HO promoter (–2720 to –1814 upstream from the ATG) was made by PCR with primers designed to generate a fragment with HinDIII and BsiWI overhang sequences. This fragment was inserted into HinDIII–BsiWI digested pUC21-NotI. A 507 bp fragment (HO-R) with sequences from the 3′ end of the HO gene (+1199 to +1699 downstream of the ATG) was made by PCR with primers designed to generate a fragment with EcoRI and SfiI overhang sequences. This fragment was inserted into EcoRI–SfiI digested pUC21-NotI.
• Vector type: Yeast Expression ; yeast integration

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry