pUK21-NotI
(Plasmid
#51660)
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Purpose(Empty Backbone) Bacterial cloning vector based on pUK21 (Kan)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51660 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUK21deltaBB
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Backbone manufacturerStillman Lab, Addgene plasmid # 51658
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Modifications to backbonePlasmid pUK21-NotI was constructed by inserting the 123 bp NotI polylinker fragment from plasmid pFA6b into pUK21deltaBB that had been cleaved with NotI.
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUK21-NotI was a gift from David Stillman (Addgene plasmid # 51660 ; http://n2t.net/addgene:51660 ; RRID:Addgene_51660) -
For your References section:
Yeast vectors for integration at the HO locus. Voth WP, Richards JD, Shaw JM, Stillman DJ. Nucleic Acids Res. 2001 Jun 15;29(12):E59-9. 10.1093/nar/29.12.e59 PubMed 11410682
