pUK21-deltaBB
(Plasmid #51658)

• Purpose: (Empty Backbone) Bacterial cloning vector based on pUK21 (Kan)
• Depositing Lab: David Stillman
• Publication: Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.

Backbone

• Vector backbone: pUK21
• Modifications to backbone: pUK21 was digested with BamHI and BglII followed by ligation to delete the fragment between the two sites; the resulting plasmid has neither a BamHI nor a BglII restriction site.
• Vector type: Cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry