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Addgene

Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)
(Plasmid #51656)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51656 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentivirus backbone
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Quasar2-mOrange-Chr64-GFP
  • Alt name
    Arch3.77H97Q
  • Species
    Synthetic
  • Insert Size (bp)
    3349
  • Promoter human synapsin
  • Tags / Fusion Proteins
    • mOrange
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer AGTCGTGTCGTGCCTGAGAG
  • 3′ sequencing primer GCAGCGTATCCACATAGCGTAAA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP) was a gift from Adam Cohen (Addgene plasmid # 51656 ; http://n2t.net/addgene:51656 ; RRID:Addgene_51656)
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