APP-bio-His
(Plasmid #51643)

• Purpose: Expresses full-length Amyloid beta A4 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
• Depositing Lab: Gavin Wright
• Publication: Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6618
• Total vector size (bp): 8735
• Modifications to backbone: Rat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: APP
• Alt name: Amyloid beta A4 protein precursor
• Species: H. sapiens (human)
• Insert Size (bp): 2117
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Promoter: CMV
• Tags / Fusion Proteins:
  • rat CD4 d3+4 (C terminal on backbone)
  • enzymatic biotinylation sequence (C terminal on backbone)
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert was chemically synthesised.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Article ref 8