pCDH-3xFLAG-TERT
(Plasmid #51631)

• Purpose: Mammalian expression of 3xFLAG tagged TERT, N-terminal.
• Depositing Lab: Steven Artandi
• Publication: Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012.

Backbone

• Vector backbone: pCDH
• Backbone manufacturer: unknown
• Modifications to backbone: N terminal 3xFLAG tag after Kozak
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TERT
• Species: H. sapiens (human)
• Insert Size (bp): 3399
• GenBank ID:
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry