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Addgene

pCDNA-3xFLAG-TCAB1
(Plasmid #51630)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/LIfe Technologies
  • Backbone size w/o insert (bp) 5500
  • Modifications to backbone
    N terminal 3xFLAG tag after Kozak
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCAB1
  • Alt name
    WRAP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1647
  • GenBank ID
    NM_001143990
  • Entrez Gene
    WRAP53 (a.k.a. DKCB3, TCAB1, WDR79)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer pCMV promoter forward
  • 3′ sequencing primer bGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA-3xFLAG-TCAB1 was a gift from Steven Artandi (Addgene plasmid # 51630 ; http://n2t.net/addgene:51630 ; RRID:Addgene_51630)

  • For your References section:

    A human telomerase holoenzyme protein required for Cajal body localization and telomere synthesis. Venteicher AS, Abreu EB, Meng Z, McCann KE, Terns RM, Veenstra TD, Terns MP, Artandi SE. Science. 2009 Jan 30;323(5914):644-8. doi: 10.1126/science.1165357. 10.1126/science.1165357 PubMed 19179534
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