pREDTKI
(Plasmid #51628)

• Purpose: E coli Genetic Modification
• Depositing Lab: Sheng Yang
• Publication: Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18.

Backbone

• Vector backbone: pKD46
• Backbone size w/o insert (bp): 6300
• Modifications to backbone: replace amp to kan
• Vector type: Bacterial Expression ; Red recombinase and I-SceI endonuclease

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Culture the plate in 30 C or 28 C for 24h or up to 48h to see if any colonies appear. LB +1% glucose plate, or SOC plate improve growth.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: I-SceI
• Alt name: mitochondrial intron of Saccharomyces cerevisiae
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3000
• Entrez Gene: SCEI (a.k.a. Q0160)
• Promoter: trc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • pREDTKI
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: pUC19RP12 Nucl. Acids Res. (1999) 27 (22): 4409-4415. György Pósfai, Institute of Biochemistry, Biological Research Center, H-6701 Szeged, Hungary
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry