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PurposeE. coli genome modification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51625 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepKD46
- Backbone size w/o insert (bp) 6300
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Vector typeBacterial Expression ; Red recombinase and I-SceI endonuclease
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsCulture the plate in 30 C or 28 C for 24h or up to 48h to see if any colonies appear. LB +1% glucose plate, or SOC plate improve growth.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameI-SceI
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Alt namemitochondrial intron of Saccharomyces cerevisiae
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3000
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Entrez GeneSCEI (a.k.a. Q0160)
- Promoter pRha
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer none (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypUC19RP12 Nucl. Acids Res. (1999) 27 (22): 4409-4415. György Pósfai, Institute of Biochemistry, Biological Research Center, H-6701 Szeged, Hungary
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREDIA was a gift from Sheng Yang (Addgene plasmid # 51625 ; http://n2t.net/addgene:51625 ; RRID:Addgene_51625) -
For your References section:
High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. 10.1128/AEM.00313-14 PubMed 24747889