pME-basGal80
(Plasmid
#51621)
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Purposevertebrate expression of Gal80 preceded by a basal promoter
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneGateway middle entry clone (pME)
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Vector typevertebrate expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal80
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SpeciesS. cerevisiae (budding yeast)
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GenBank ID
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Entrez GeneGAL80 (a.k.a. YML051W)
- Promoter basal promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-basGal80 was a gift from Joshua Bonkowsky (Addgene plasmid # 51621 ; http://n2t.net/addgene:51621 ; RRID:Addgene_51621) -
For your References section:
Gal80 intersectional regulation of cell-type specific expression in vertebrates. Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. 10.1002/dvdy.22734 PubMed 21905164