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Addgene

pME-Gal80
(Plasmid #51620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Gateway middle entry clone (pME)
  • Vector type
    vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal80
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
    NM_001182409.1
  • Entrez Gene
    GAL80 (a.k.a. YML051W)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pME-Gal80 was cloned by PCR from TubP-GAL80 (Lee and Luo, 1999; obtained from Addgene, plasmid #17748) using primers 5'-ATGGACTACAACAAGAGATCTTCG-3' and 5'-TTATAAACTATAATGCGAGATATTGCT-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-Gal80 was a gift from Joshua Bonkowsky (Addgene plasmid # 51620 ; http://n2t.net/addgene:51620 ; RRID:Addgene_51620)

  • For your References section:

    Gal80 intersectional regulation of cell-type specific expression in vertebrates. Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. 10.1002/dvdy.22734 PubMed 21905164
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