pME-nlsGal80(opt)
(Plasmid #51618)

• Purpose: vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop
• Depositing Lab: Joshua Bonkowsky
• Publication: Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8.

Backbone

• Vector backbone: Gateway middle entry clone (pME)
• Vector type: vertebrate expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gal80(opt)
• Alt name: Gal80
• Species: S. cerevisiae (budding yeast)
• Mutation: codon optimized
• Entrez Gene: GAL80 (a.k.a. YML051W)
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pME-NLS-Gal80opt was generated by PCR using primers 5'-ATGGCTCCAAAGAAGAAGCGTAAGGTAATGGACTACAACAAAAGGAGCAG-3' for the nuclear localization signal (Kwan et al., 2007) and 5'-CAGTGAGTAGTGAGAGATATTTG-3' for codon-optimized Gal80 (GenScript; GenBank accession no. JN314417).