NS5A-mCherry
(Plasmid #51616)

• Purpose: Hep C non-structural protein 5A (binds PI4KA)
• Depositing Lab: Tamas Balla
• Publication: Hammond et al J Cell Biol. 2014 Apr 7.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 4723
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NS5A
• Species: Hepatitis C
• Insert Size (bp): 1341
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Xin Wang via addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry