iRFP-FRB-Rab5
(Plasmid #51612)

• Purpose: Recruiter to Rab5-positive endosomes
• Depositing Lab: Tamas Balla
• Publication: Hammond et al J Cell Biol. 2014 Apr 7.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4902
• Modifications to backbone: iRFP replaces EGFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: FRB
• Alt name: FKBP12-rapamycin binding domain
• Species: H. sapiens (human)
• Insert Size (bp): 279
• Promoter: CMV
• Tag / Fusion Protein:
  • iRFP (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-R

Gene/Insert 2

• Gene/Insert name: Rab5
• Species: Canis familiaris
• Insert Size (bp): 648

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: iRFP was from Vladislav Verkhusha via Addgene
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry