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PurposeExpress DsRed monomer-tagged hAIM2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51609 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCVpuro
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 6264
- Total vector size (bp) 8009
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAIM2
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SpeciesH. sapiens (human)
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Insert Size (bp)1029
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Entrez GeneAIM2 (a.k.a. PYHIN4)
- Promoter MESV
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Tag
/ Fusion Protein
- DsRed monomer (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer 5' MSCV primer
- 3′ sequencing primer 3' MSCV primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVpuro-hAIM2-DsRed monomer was a gift from Emad Alnemri (Addgene plasmid # 51609 ; http://n2t.net/addgene:51609 ; RRID:Addgene_51609) -
For your References section:
AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676