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FRIG-myc-Sema4DdeltaC
(Plasmid #51607)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FRIG
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 9300
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sema4DdeltaC
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Mutation
    deleted last 70 amino acids in the C-terminus
  • GenBank ID
    NM_001281880
  • Entrez Gene
    Sema4d (a.k.a. CD100, Semacl2, Semaj, Semcl2, coll-4)
  • Promoter RSV
  • Tag / Fusion Protein
    • myc tag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TGTCAAGTTCAGGTGGTCACAGG
  • 3′ sequencing primer TACAACTGCTACAAGGGCTAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRIG-myc-Sema4DdeltaC was a gift from Suzanne Paradis (Addgene plasmid # 51607 ; http://n2t.net/addgene:51607 ; RRID:Addgene_51607)

  • For your References section:

    Sema4D localizes to synapses and regulates GABAergic synapse development as a membrane-bound molecule in the mammalian hippocampus. Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S. Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10. 10.1016/j.mcn.2013.08.004 PubMed 24036351
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