pSuper-Rem2-2
(Plasmid
#51595)
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PurposeshRNA 2 against rodent Rem2
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSuper
- Backbone size w/o insert (bp) 4000
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Vector typeRNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSuper-Rem2-2 was a gift from Suzanne Paradis (Addgene plasmid # 51595 ; http://n2t.net/addgene:51595 ; RRID:Addgene_51595) -
For your References section:
A loss-of-function analysis reveals that endogenous Rem2 promotes functional glutamatergic synapse formation and restricts dendritic complexity. Moore AR, Ghiretti AE, Paradis S. PLoS One. 2013 Aug 26;8(8):e74751. doi: 10.1371/journal.pone.0074751. eCollection 2013. 10.1371/journal.pone.0074751 PubMed 23991227