PhyB-mCherry-Spc72
(Plasmid #51582)

• Purpose: Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae
• Depositing Lab: Chao Tang
• Publication: Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub 2013 Jun 12.

Backbone

• Vector backbone: pRS305
• Backbone size w/o insert (bp): 7236
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PhyB
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 6901
• Mutation: 1-908aa only
• Entrez Gene: PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
• Promoter: ADH1pr
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • Spc72 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Apa1 (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: mCherry F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jared Toettcher

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.