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Addgene

linker-mCitrine-PIF6-NAT
(Plasmid #51576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    F6A-NAT
  • Backbone size w/o insert (bp) 3657
  • Vector type
    Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIF6
  • Alt name
    PIL2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1113
  • Mutation
    1-100aa of PIF6
  • Entrez Gene
    PIL2 (a.k.a. AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2)
  • Promoter none
  • Tags / Fusion Proteins
    • linker (N terminal on insert)
    • mCitrine (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jared Toettcher

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the 11-aa linker is AAAGDGAGLIN

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    linker-mCitrine-PIF6-NAT was a gift from Chao Tang (Addgene plasmid # 51576 ; http://n2t.net/addgene:51576 ; RRID:Addgene_51576)

  • For your References section:

    A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Yang X, Jost AP, Weiner OD, Tang C. Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub 2013 Jun 12. 10.1091/mbc.E13-03-0126 PubMed 23761071
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