pTXB1-sfGFP
(Plasmid #51562)

• Purpose: Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
• Depositing Labs: Sankar Adhya, Francisco Malagon
• Publication: Plamids for protein purification in E. coli of superfolder GFP, CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda phage) (unpublished)

Backbone

• Vector backbone: pTXB1
• Backbone manufacturer: NEB
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Expression of the insert requires retransformation into a T7 RNA polymerase containing strain
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: superfolder GFP
• Species: Synthetic
• Tag / Fusion Protein:
  • Intein-Chiting Binding Domanin (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry