pBAD24-rnc-sfGFP
(Plasmid
#51560)
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PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMaintained in DH5alpha, assayed in MG1655 after retransformation.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namernc
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Alt nameRNase III
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SpeciesE. coli
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MutationContains additonal aa at the end of the sfGFP in the N and C terminus (in frame with the polylinker)
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Entrez Genernc (a.k.a. b2567, ECK2565)
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Tags
/ Fusion Proteins
- superfolder GFP (C terminal on insert)
- GLESTCRHASLAVLADERRFSA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD24-rnc-sfGFP was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51560 ; http://n2t.net/addgene:51560 ; RRID:Addgene_51560) -
For your References section:
RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli. Malagon F. RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26. 10.1261/rna.038588.113 PubMed 23893733
Map uploaded by the depositor.
