pBAD24-rnc-sfGFP
(Plasmid #51560)

• Purpose: Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).
• Depositing Labs: Sankar Adhya, Francisco Malagon
• Publication: Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26.

Backbone

• Vector backbone: pBAD24
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Maintained in DH5alpha, assayed in MG1655 after retransformation.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rnc
• Alt name: RNase III
• Species: E. coli
• Mutation: Contains additonal aa at the end of the sfGFP in the N and C terminus (in frame with the polylinker)
• Entrez Gene: rnc (a.k.a. b2567, ECK2565)
• Tags / Fusion Proteins:
  • superfolder GFP (C terminal on insert)
  • GLESTCRHASLAVLADERRFSA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry