pBAD24-sfGFPx2
(Plasmid #51559)

• Purpose: Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
• Depositing Labs: Sankar Adhya, Francisco Malagon
• Publication: Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26.

Backbone

• Vector backbone: pBAD24
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Maintained in DH5alpha, assayed in MG1655 or BW25113 after retransformation (fluorescence intensity for this plasmid is background dependent).
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: superfolder GFP
• Species: Synthetic
• Mutation: Contains additonal aa in the N and C terminus (in frame with the polylinker)
• Tags / Fusion Proteins:
  • MARARA (N terminal on insert)
  • GLESTCRHASLAVLADERRFSA (C terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sfGFP in this plasmid contains Y148C and K214I mutations that do not affect the function of the plasmid.