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Addgene

pBAD24-sfGFPx1
(Plasmid #51558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Maintained in DH5alpha, assayed in MG1655 or BW25113 after retransformation (fluorescence intensity for this plasmid is background dependent).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    superfolder GFP
  • Species
    Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned by Gene Synthesis (Excellgen, Rockville,MD) for our lab
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD24-sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558 ; http://n2t.net/addgene:51558 ; RRID:Addgene_51558)

  • For your References section:

    RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli. Malagon F. RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26. 10.1261/rna.038588.113 PubMed 23893733
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