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PurposeDICE donor plasmid with the FOXA2 and OTX2 transcription factors.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51551 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2attPC
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameFOXA2
Gene/Insert 2
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Gene/Insert nameOTX2
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2attPC-FO was a gift from Michele Calos (Addgene plasmid # 51551 ; http://n2t.net/addgene:51551 ; RRID:Addgene_51551) -
For your References section:
DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, Chen-Tsai Y, Calos MP. Nucleic Acids Res. 2013 Dec 4. 10.1093/nar/gkt1290 PubMed 24304893