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Purposep2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep2attNG
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Vector typeMammalian Expression ; Human Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Growth instructionsThis plasmid may be difficult to grow and a strain such as MDS42 ΔrecA (Scarab Genomics C-0185) might increase yields.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP4m
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2attNG-H11-long was a gift from Michele Calos (Addgene plasmid # 51545 ; http://n2t.net/addgene:51545 ; RRID:Addgene_51545) -
For your References section:
DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, Chen-Tsai Y, Calos MP. Nucleic Acids Res. 2013 Dec 4. 10.1093/nar/gkt1290 PubMed 24304893
Map uploaded by the depositor.
