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pDsRed-monomer-hAIM2
(Plasmid #51544)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51544 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDsRed-Monomer
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3313
  • Total vector size (bp) 4320
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AIM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1029
  • Entrez Gene
    AIM2 (a.k.a. PYHIN4)
  • Promoter lac operator
  • Tag / Fusion Protein
    • DsRed monomer (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HinIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer 5' DsRed primer
  • 3′ sequencing primer Aim2 internal primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDsRed-monomer-hAIM2 was a gift from Emad Alnemri (Addgene plasmid # 51544 ; http://n2t.net/addgene:51544 ; RRID:Addgene_51544)

  • For your References section:

    AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES. Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21. 10.1038/nature07710 PubMed 19158676
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