pEGFP-N1-hAIM2
(Plasmid #51542)

• Purpose: Express GFP-tagged hAIM2 in mammalian cells
• Depositing Lab: Emad Alnemri
• Publication: Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub 2009 Jan 21.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 5732
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AIM2
• Species: H. sapiens (human)
• Insert Size (bp): 1029
• Entrez Gene: AIM2 (a.k.a. PYHIN4)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pEGFP-N1 5' primer
• 3′ sequencing primer: Aim2 internal primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry